标題：The response of carbohydrate metabolism to the fluctuation of relative humidity (RH) in the desert soil cyanobacterium Phormidium tenue

作者：Chen, LZ(Chen, Lanzhou);Yang, Y(Yang, Yan);Deng, SD(Deng, Songdiang);Xu, YH(Xu, Yanhong);Wang, GH(Wang, Gaohong);Liu, YD(Liu, Yongding)

來源出版物：EUROPEAN JOURNAL OF SOIL BIOLOGY 卷：48 頁：11-16 出版年：JAN-FEB 2012

摘要：The excreting of exopolysaccharides (EPS) is thought as one of the main protection ways for cyanobacteria in desert algal crusts to survive desiccation. But how cyanobacteria adjust their carbohydrate metabolism to survive this stress is not elucidated. In this study, we treated Phormidium tenue, a cyanobacterium isolated from biological soil crusts with the changes of relative humidity (RH) to simulate different levels of desiccation and investigated its carbohydrate metabolism. It was found that photosynthetic activity (Fv/Fm) and cellular total carbohydrates production decreased significantly at low RH. But the production of EPS, reducing sugar, sucrose and the activity of sucrose phosphate synthase (SPS) increased significantly at low RH and reached maximum at 75% RH. Low RH could also cause the enhanced production of reactive oxygen species (ROS) generation, malondialdehyde (MDA) production and DNA strand breaks. However, when pretreated with exogenous 100 mg/L EPS, Fv/Fm and carbohydrate production were improved significantly, while ROS generation, MDA production and DNA strand breaks decreased significantly at various levels of RH in P tenue. These results indicated that P. tenue in arid regions could enhance desiccation tolerance by adjusting the carbohydrate metabolism to eliminate ROS and decrease oxidative damage.

文獻類型：Article

語種：English

作者關鍵詞：Biological soil crusts; Exoplysaccharides (EPS); Desiccation; Oxidative damage

擴展關鍵詞：DESICCATION TOLERANCE; MICROCOLEUS-VAGINATUS; OXIDATIVE DAMAGE; SALT STRESS; DNA-DAMAGE; EXOPOLYSACCHARIDES; CRUSTS; POLYSACCHARIDES; PHOTOSYNTHESIS; INVOLVEMENT

通訊作者地址：Chen, LZ (通訊作者),Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China

地址：

[Chen, Lanzhou; Yang, Yan; Deng, Songdiang; Xu, Yanhong]Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China

[Wang, Gaohong; Liu, Yongding]Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China

電子郵件地址：chenlanzhou@yahoo.com.cn

學科類别：Environmental Sciences & Ecology; Agriculture

ISSN：1164-5563