标題: Chitosan and collagen layer-by-layer assembly modified oriented nanofibers and their biological properties

作者: Li, D (Li, Dan); Dai, FF (Dai, Fangfang); Li, H (Li, Hao); Wang, C (Wang, Chi); Shi, XW (Shi, Xiaowen); Cheng, YX (Cheng, Yanxiang); Deng, HB (Deng, Hongbing)

來源出版物: CARBOHYDRATE POLYMERS  卷: 254  文獻号: 117438  DOI: 10.1016/j.carbpol.2020.117438  出版年: FEB 15 2021  

摘要: Layer-by-layer self-assembly (LBL) is an effective method to prepare potential biomaterial with multilayer coatings, and few reports have focused on the variation of oriented microstructure during LBL process. In this study, polycaprolactone (PCL) and type collagen (COL) were electrospun to oriented nanofibrous mats, and chitosan (CS) and COL molecules were then deposited on the mats by LBL technique. Zeta potential, FT-IR analysis and XPS measurement indicated the successful fabrication and modification. Changes in surface morphology and increase in surface roughness were observed in LBL process. Additionally, LBL-structured mats exhibited improved mechanical properties with the maximal tensile strength of 35.1 +/- 7.0 MPa and the best elongation of 106.0 +/- 11.5 %. CCK-8 and live/dead assays illustrated that the cell viability of the mats increased more than 20 % after LBL modification. More importantly, cells seeded onto the mats showed oriented adhesion and growth along the direction of nanofiber arrangement in LBL modified mats, which provided an effective strategy for realizing the controlled growth of cells.

入藏号: WOS:000604615200008

PubMed ID: 33357911

語言: English

文獻類型: Article

作者關鍵詞: Oriented nanofibers; Multilayer coating structure; Chitosan; Collagen; Biocompatibility

地址: [Li, Dan; Li, Hao; Wang, Chi; Shi, Xiaowen; Deng, Hongbing] Wuhan Univ, Sch Resource & Environm Sci, Hubei Engn Ctr Nat Polymers Based Med Mat,Hubei K, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

[Dai, Fangfang; Cheng, Yanxiang] Wuhan Univ, Dept Obstet & Gynecol, Renmin Hosp, Wuhan 430060, Hubei, Peoples R China.

通訊作者地址: Deng, HB (通訊作者)，Wuhan Univ, Sch Resource & Environm Sci, Hubei Engn Ctr Nat Polymers Based Med Mat,Hubei K, Hubei Int Sci & Technol Cooperat Base Sustainable, Wuhan 430079, Peoples R China.

電子郵件地址: hbdeng@whu.edu.cn

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